Tech Tips & Posters

Mesenchymal Stem Cell Culture Under SF, XF Conditions - Useful Tips

How to isolate mesenchymal stem cells from tissue, what reagents to use while seeding the mesenchymal stem cells, and how to sub-culture mesenchymal stem cells under serum-free, xeno-free culture conditions.

Serum-free Freezing Medium - Performance Validation

Read here why it is important to cryopreserve cells in a serum-free freezing medium while using medium free of serum in the cell culture.

A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources

A scientific poster presents the development of novel serum-free, xeno-free media and supplements that efficiently differentiate hMSCs from various sources into adipocytes, osteoblasts, and chondrocytes. Molecular characterization and functional assays were used to evaluate the quality and purity of the differentiated cells.

hMSC Serum-Free, Xeno-Free Culture System

A scientific poster that presents a novel Serum-Free, Xeno-Free culture system for human Mesenchymal Stem Cells, suitable for therapeutic applications. The system was developed and evaluated by BI's R&D department.

Introduction to Fetal Bovine Serum Class

Everything you wanted to know about fetal bovine serum in cell culture: What grades of FBS are available? What tests are performed for Quality Control?

Single-strength Medium

Read here instructions for preparation of single-strength medium.

Powdered Medium

Read here instructions for preparation of powdered medium.

Mycoplasma: Effective Detection and Treatment

Read here how to use PCR for the detection of mycoplasma in cell cultures and what are the methods for treating contaminated cultures.

10 Quick Tips to Prevent Mycoplasma Contamination

Follow these quick, easy tips to prevent cell culture contamination

Guide for Antibiotics Selection

Read here a guide for antibiotics selection and appropriate concentrations

Serum-Free Medium for Adherent Cells

In order to succeed in the serum-free growth of adherent cells, it is necessary to follow proper procedures carefully. Read here detailed  instructions and techniques.

Serum-Free Medium for CHO Cells

Read here the technical guide for a successful serum-free culture of CHO cells

Serum-Free Medium for Animal Cell Culture

A thorough technical guide for the use of serum-free media and supplement in animal cell culture in suspension