Stem Cell Media Selection Guide

Which stem cell culture medium is right for you?

Medium

NutriStem® hPSC XF Medium
NutriStem® hPSC XF Medium, Modified GF-Free

NutriStem® V9 XF Medium

MSC NutriStem® XF Medium
Cell Type Human PSCs Human PSCs Human PSCs Human PSCs Human PSCs Human PSCs Human MSCs Human MSCs
Application Feeder-Dependent hPSC culture Feeder-Free
hPSC culture
Translational hPSC culture Translational hPSC culture, single cell Reprogramming hPSC culture on vitronectin Completely Defined Translational hMSC culture Translational hMSC culture
Additional Supplement None None None None bFGF (20 ng/mL) NutriStem® V9 ACF Supplement Mix MSC NutriStem® Supplement Mix PLTMax® Human Platelet Lysate
Matrix MEFs/HFFs Matrigel
Cultrex
Geltrex
Other
Vitronectin
LaminStem™ 521
Laminin-511
LaminStem™ 521 LaminStem™ 521
Matrigel
Laminin-511
Vitronectin MSC Attachment Solution None
Xeno-Free Possible (HFFs) No Yes Yes Yes Yes Yes Yes
Weekend-Free Feeding* No Yes Yes Yes n/a Yes n/a n/a
Dissociation Reagent Collagenase IV Dispase
Collagenase IV
Accutase
0.5mM EDTA
0.5mM EDTA
Accutase
Trypsin-EDTA
Accutase
Trypsin-EDTA
Accutase
0.5mM EDTA Recombinant Trypsin Solution Recombinant Trypsin Solution
Freezing Media CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
MSC Freezing Solution MSC Freezing Solution
Additional Reagents Gelatin, Human Serum Albumin (HSA), Soybean Trypsin Inhibitor, EZ-PCR™ Mycoplasma Detection Kit, DPBS, Aquaguard™-1 Solution, Aquaguard™-2 Solution for Water Baths

*Weekend-free feeding is possible, though with any hPSC culture, optimal growth is seen with daily feeding. It is not recommended to skip feedings on a routine or long-term basis.