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Stem Cell Media Selection Guide

Which stem cell culture medium is right for you?

Medium

NutriStem® hPSC XF Medium
NutriStem® hPSC XF Medium, Modified GF-Free
MSC NutriStem® XF Medium
Cell Type Human PSCs Human PSCs Human PSCs Human PSCs Human PSCs Human MSCs Human MSCs
Application Feeder-Dependent hPSC culture Feeder-Free
hPSC culture
Translational hPSC culture Translational hPSC culture, single cell Reprogramming Completely Defined Translational hMSC culture Translational hMSC culture
Additional Supplement None None None None bFGF (20 ng/mL) MSC NutriStem® Supplement Mix PLTMax® Human Platelet Lysate
Matrix MEFs/HFFs Matrigel
Cultrex
Geltrex
Other
Vitronectin
LaminStem™ 521
Laminin-511
LaminStem™ 521 LaminStem™ 521
Matrigel
Laminin-511
MSC Attachment Solution None
Xeno-Free Possible (HFFs) No Yes Yes Yes Yes Yes
Weekend-Free Feeding* No Yes Yes Yes n/a n/a n/a
Dissociation Reagent Collagenase IV Dispase
Collagenase IV
Accutase
0.5mM EDTA
0.5mM EDTA
Accutase
Trypsin-EDTA
Accutase
Trypsin-EDTA
Accutase
Recombinant Trypsin Solution Recombinant Trypsin Solution
Freezing Media NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
NutriFreez® D10
Cryopreservation Medium
Additional Reagents Gelatin, Human Serum Albumin (HSA), Soybean Trypsin Inhibitor, EZ-PCR™ Mycoplasma Detection Kit, DPBS, Aquaguard™-1 Solution, Aquaguard™-2 Solution for Water Baths

*Weekend-free feeding is possible, though with any hPSC culture, optimal growth is seen with daily feeding. It is not recommended to skip feedings on a routine or long-term basis.