RPMI 1640 Medium, no glutamine, with Sodium Bicarbonate

Catalog Number: 01-104-1A

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium nitrate (Ca(NO3)2 4H2O) 100.00
Potassium chloride (KCl) 400.00
Magnesium sulfate (MgSO4 ) 48.84
Sodium chloride (NaCl) 6000.00
Sodium bicarbonate (NaHCO3) 2000.00
Sodium Phosphate (Na2HPO4) 800.85
OTHER COMPONENTS      
Glucose 2000.00
Glutathione Reduced 1.00
Phenol red 5.00
AMINO ACIDS  
L-Arginine 200.00
L-Asparagine-H20 50.00
L-Aspartic Acid 20.00
L-Cystine 50.00
L-Glutamic Acid 20.00
Glycine 10.00
L-Histidine 15.00
L-Hydroxyproline 20.00
L-Isoleucine 50.00
L-Leucine 50.00
L-Lysine hydrochloride 40.00
L-Methionine 15.00
L-Phenylalanine 15.00
L-Proline 20.00
L-Serine 30.00
L-Threonine 20.00
L-Tryptophan 5.00
L-Tyrosine 20.00
L-Valine 20.00
VITAMINS     
Biotin 0.20
D-Calcium Pantothenate 0.25
Choline Chloride 3.00
Folic Acid 1.00
i-Inositol 35.00
Niacinamide 1.00
p-Aminobenzoic Acid (PABA) 1.00
Pyridoxine HCl 1.00
Riboflavin 0.20
Thiamine HCl 1.00
Vitamin B12 0.005

 

Reference:
Moore, G.E., Gerner, R.E. and Franklin, H.A. (1967) J.A.M.A., 199:519.