Medium M-199 Earle`s Salts Base

Catalog Number: 01-080-1, 01-080-1A, 01-080-1B

This formulation is with L-Glutamine and Sodium Bicarbonate.

Components

Concentration (mg/L)

INORGANIC SALTS
Calcium chloride (CaCl2)(anhyd.) 200.00
Ferric nitrate (Fe(NO3)3-9H2O) 0.72
Potassium chloride (KCl) 400.00
Magnesium sulfate (MgSO4) 97.67
Sodium chloride (NaCl) 6800.00
Sodium bicarbonate (NaHCO3) 2200.00
Sodium phosphate, mono. (NaH2PO4-H2O) 140.00
OTHER COMPOUNDS  
Adenine sulfate 10.00
Adenosine 5'-triphosphate,disodium salt 1.00
Adenylic Acid 0.20
Cholesterol 0.20
Deoxyribose 0.50
D-Glucose 1000.00
Glutathione (reduced) 0.05
Guanine hydrochloride 0.30
Hypoxanthine-Na 0.40
Phenol red 20.00
Ribose 0.50
Sodium acetate 50.00
Thymine (5-Methyluracil) 0.30
Tween 80 20.00
Uracil 0.30
Xanthine-Na 0.34
AMINO ACIDS  
L-Alanine 25.00
L-Arginine hydrochloride 70.00
L-Aspartic acid 30.00
L-Cysteine HCl-H2O 0.11
L-Cystine 20.00
L-Glutamic Acid 75.00
L-Glutamine 100.00
Glycine 50.00
L-Histidine HCl H2O 21.88
L-Hydroxyproline 10.00
L-Isoleucine 20.00
L-Leucine 60.00
L-Lysine hydrochloride 70.00
L-Methionine 15.00
L-Phenylalanine 25.00
L-Proline 40.00
L-Serine 25.00
L-Threonine 30.00
L-Tryptophan 10.00
L-Tyrosine 40.00
L-Valine 25.00
VITAMINS  
Ascorbic acid 0.050
alpha-Tocopherol Phosphate 0.010
d-Biotin 0.010
Calciferol (Vitamin D2) 0.100
D-Calcium pantothenate 0.010
Choline chloride 0.500
Folic acid 0.010
i-Inositol 0.050
Menadione (Vitamin K3) 0.010
Niacin 0.025
Niacinamide 0.025
Para-aminobenzoic acid 0.050
Pyridoxal hydrochloride 0.025
Pyridoxine hydrochloride 0.025
Riboflavin 0.010
Thiamine hydrochloride 0.010
Vitamin A (acetate) 0.100