Leibovitz L-15 Medium

Catalog Number: 01-115-1A

This formulation is with L-Glutamine

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium chloride (CaCl2) 140.00
Potassium chloride (KCl) 400.00
Potassium phosphate monobasic (KH2PO4) 60.00
Magnesium chloride (MgCl2) 93.70
Magnesium sulfate (MgSO4) 97.67
Sodium chloride (NaCl) 8000.00
Sodium phosphate, dibasic (Na2HPO4) 190.00
OTHER COMPOUNDS  
D+ Galactose 900.00
Sodium pyruvate 550.00
Phenol Red 10.00
AMINO ACIDS  
L-Alanine 225.00
L-Arginine 500.00
L-Asparagine 250.00
L-Cysteine 120.00
L-Glutamine 300.00
Glycine 200.00
L-Histidine 250.00
L-Isoleucine 125.00
L-Leucine 125.00
L-Lysine 75.00
L-Methionine 75.00
L-Phenylalanine 125.00
L-Serine 200.00
L-Threonine 300.00
L-Tryptophan 20.00
L-Tyrosine 300.00
L-Valine 100.00
VITAMINS  
D-Calcium pantothenate 1.00
Choline chloride 1.00
Folic acid 1.00
i-Inositol 2.00
Niacinamide 1.00
Pyridoxine hydrochloride 1.00
Riboflavin 5'-phosphate, Na 0.10
Thiamine hydrochloride 1.00

Reference:
Leibovitz, A. (1963) Am. J. Hygiene 78:173