Grace's Insect Medium

Catalog Number: 01-155-1A

This formulation is with L-Glutamine.

Components

Concentration (mg/L)

INORGANIC SALTS
Calcium chloride (CaCl2) 1000.00
Magnesium chloride (MgCl2-6H20) 2280.00
Magnesium sulfate (MgSO4-7H20) 2780.00
Potassium chloride (KCl) 2240.00
Sodium bicarbonate (NaHCO3) 350.00
Sodium phosphate, mono. (NaH2PO4-H2O) 1013.00
OTHER COMPONENTS
D-Fructose 400.00
Fumaric acid 55.00
D-Glucose 700.00
Alpha-Ketoglutaric acid 370.00
Malic acid 670.00
Succinic acid 60.00
Sucrose 26680.00
AMINO ACIDS
beta-Alanine 200.00
L-Alanine 225.00
L-Arginine hydrochloride 700.00
L-Asparagine 350.00
L-Aspartic acid 350.00
L-Cystine 22.00
L-Glutamic acid 600.00
L-Glutamine 600.00
Glycine 650.00
L-Histidine 2500.00
L-Isoleucine 50.00
L-Leucine 75.00
L-Lysine hydrochloride 625.00
L-Methionine 50.00
L-Phenylalanine 150.00
L-Proline 350.00
DL-Serine 1100.00
L-Threonine 175.00
L-Tryptophan 100.00
L-Tyrosine 50.00
L-Valine 100.00
VITAMINS
Biotin 0.01
D-Calcium pantothenate 0.02
Choline chloride 0.20
Folic acid 0.02
i-Inositol 0.02
Nicotinic acid (Niacin) 0.02
Para-aminobenzoic acid 0.02
Pyridoxine hydrochloride 0.02
Riboflavin 0.02
Thiamine hydrochloride 0.02
Reference:
Grace, T.C.C., Nature, 195:788 (1962).