F-12 Nutrient Mixture (Ham's)

Catalog Number: 01-095-1A

This formulation is with L-Glutamine

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium chloride (Anhydrous) 33.22
Cupric sulfate (CuSO4-5H2O) 0.0025
Ferric sulfate (FeSO4-7H2O) 0.834
Potassium chloride (KCl) 223.60
Magnesium chloride (Anhydrous) 57.22
Sodium chloride (NaCl) 7599.00
Sodium bicarbonate (NaHCO3) 1176.00
Sodium phosphate, dibas (Anhydrous) 142.04
Zinc sulfate (ZnSO4-7H2O) 0.863
OTHER COMPONENTS  
D-Glucose 1802.00
Hypoxanthine Na 4.77
Linoleic Acid 0.084
Lipoic Acid 0.21
Phenol red 1.20
Putrescine-2HCl 0.161
Sodium Pyruvate 110.00
Thymidine 0.73
AMINO ACIDS  
L-Alanine 8.90
L-Arginine hydrochloride 211.00
L-Asparagine-H2O 15.00
L-Aspartic acid 13.30
L-Cysteine-HCl-H2O 35.12
L-Glutamic acid 14.70
L-Glutamine 146.00
Glycine 7.50
L-Histidine-HCl-H2O 20.96
L-Isoleucine 3.94
L-Leucine 13.10
L-Lysine hydrochloride 36.50
L-Methionine 4.48
L-Phenylalanine 4.96
L-Proline 34.50
L-Serine 10.50
L-Threonine 11.90
L-Tryptophan 2.04
L-Tyrosine 5.40
L-Valine 11.70
VITAMINS  
Biotin 0.0073
D-Calcium pantothenate 0.48
Choline chloride 13.96
Folic acid 1.30
i-Inositol 18.00
Niacinamide 0.037
Pyridoxine hydrochloride 0.062
Riboflavin 0.038
Thiamine hydrochloride 0.34
Vitamin B12 1.36

Reference:
Ham, R. G. (1965) Proc. Nat. Acad. Sci., 53, 288.