F-10 Nutrient Mixture (Ham's)

Catalog Number: 01-090-1A, 01-090-1B

This formulation is with L-Glutamine

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium chloride (CaCl2) 33.30
Cupric sulfate (CuSO4-5H2O) 0.0025
Ferrous sulfate (FeSO4-7H2O) 0.834
Potassium chloride (KCl) 285.00
Potassium phosphate (KH2PO4) 83.00
Magnesium sulfate (MgSO4) 74.62
Sodium chloride (NaCl) 7400.00
Sodium bicarbonate (NaHCO3) 1200.00
Sodium phosphate, dibas. (Na2HPO4) 153.70
Zinc sulfate (ZnSO4-7H2O) 0.0288
OTHER COMPONENTS  
D-Glucose 1100.00
Hypoxanthine Na 4.70
Lipoic Acid 0.20
Phenol Red 1.20
Sodium Pyruvate 110.00
Thymidine 0.70
AMINO ACIDS  
L-Alanine 9.00
L-Arginine HCl 211.00
L-Asparagine H2O 15.00
L-Aspartic acid 13.30
L-Cysteine 25.00
L-Glutamic acid 14.70
L-Glutamine 146.00
Glycine 7.51
L-Histidine HCl H2O 23.00
L-Isoleucine 2.60
L-Leucine 13.00
L-Lysine hydrochloride 29.00
L-Methionine 4.48
L-Phenylalanine 5.00
L-Proline 11.50
L-Serine 10.50
L-Threonine 3.57
L-Tryptophan 0.60
L-Tyrosine 1.80
L-Valine 3.50
VITAMINS  
Biotin 0.024
D-Calcium pantothenate 0.715
Choline chloride 0.698
Folic acid 1.32
i-Inositol 0.541
Niacinamide 0.615
Pyridoxine HCl 0.206
Riboflavin 0.376
Thiamine hydrochloride 1.00
Vitamin B12 1.36

Reference:
Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.