Catalog Number: 01-090-5A
This formulation is with L-Glutamine
INORGANIC SALTS
Components |
Concentration (mg/L) |
| Calcium chloride (CaCl2) | 333.00 |
| Cupric sulfate (CuSO4-5H2O) | 0.025 |
| Ferrous sulfate (FeSO4-7H2O) | 8.34 |
| Potassium chloride (KCl) | 2850.00 |
| Potassium phosphate (KH2PO4) | 830.00 |
| Magnesium sulfate (MgSO4) | 746.2 |
| Sodium chloride (NaCl) | 74000.00 |
| Sodium phosphate, dibas. (Na2HPO4) | 1537.00 |
| Zinc sulfate (ZnSO4-7H2O) | 0.288 |
| OTHER COMPONENTS | |
| D-Glucose | 11000.00 |
| Hypoxanthine Na | 47.00 |
| Lipoic Acid | 2.00 |
| Phenol Red | 12.00 |
| Sodium Pyruvate | 1100.00 |
| Thymidine | 7.00 |
| AMINO ACIDS | |
| L-Alanine | 90.00 |
| L-Arginine HCl | 2110.00 |
| L-Asparagine H2O | 150.00 |
| L-Aspartic acid | 133.00 |
| L-Cysteine | 250.00 |
| L-Glutamic acid | 147.00 |
| L-Glutamine | 1460.00 |
| Glycine | 75.10 |
| L-Histidine HCl H2O | 230.00 |
| L-Isoleucine | 26.00 |
| L-Leucine | 130.00 |
| L-Lysine hydrochloride | 290.00 |
| L-Methionine | 44.80 |
| L-Phenylalanine | 50.00 |
| L-Proline | 115.00 |
| L-Serine | 105.00 |
| L-Threonine | 35.70 |
| L-Tryptophan | 6.00 |
| L-Tyrosine | 18.00 |
| L-Valine | 35.00 |
| VITAMINS | |
| Biotin | 0.24 |
| D-Calcium pantothenate | 7.15 |
| Choline chloride | 6.98 |
| Folic acid | 13.20 |
| i-Inositol | 5.41 |
| Niacinamide | 6.15 |
| Pyridoxine HCl | 2.06 |
| Riboflavin | 3.76 |
| Thiamine hydrochloride | 10.00 |
| Vitamin B12 | 13.60 |
Reference:
Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.
