F-10 Nutrient Mixture (Ham's) (10X)

Catalog Number: 01-090-5A

This formulation is with L-Glutamine

INORGANIC SALTS 

Components

Concentration (mg/L)

Calcium chloride (CaCl2) 333.00
Cupric sulfate (CuSO4-5H2O) 0.025
Ferrous sulfate (FeSO4-7H2O) 8.34
Potassium chloride (KCl) 2850.00
Potassium phosphate (KH2PO4) 830.00
Magnesium sulfate (MgSO4) 746.2
Sodium chloride (NaCl) 74000.00
Sodium phosphate, dibas. (Na2HPO4) 1537.00
Zinc sulfate (ZnSO4-7H2O) 0.288
OTHER COMPONENTS  
D-Glucose 11000.00
Hypoxanthine Na 47.00
Lipoic Acid 2.00
Phenol Red 12.00
Sodium Pyruvate 1100.00
Thymidine 7.00
AMINO ACIDS  
L-Alanine 90.00
L-Arginine HCl 2110.00
L-Asparagine H2O 150.00
L-Aspartic acid 133.00
L-Cysteine 250.00
L-Glutamic acid 147.00
L-Glutamine 1460.00
Glycine 75.10
L-Histidine HCl H2O 230.00
L-Isoleucine 26.00
L-Leucine 130.00
L-Lysine hydrochloride 290.00
L-Methionine 44.80
L-Phenylalanine 50.00
L-Proline 115.00
L-Serine 105.00
L-Threonine 35.70
L-Tryptophan 6.00
L-Tyrosine 18.00
L-Valine 35.00
VITAMINS  
Biotin 0.24
D-Calcium pantothenate 7.15
Choline chloride 6.98
Folic acid 13.20
i-Inositol 5.41
Niacinamide 6.15
Pyridoxine HCl 2.06
Riboflavin 3.76
Thiamine hydrochloride 10.00
Vitamin B12 13.60

Reference:
Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.