DMEM/F-12, HEPES, Powder

Catalog Number: 11-170-1K, 11-170-1G, 11-170-1M, 11-170-1N

This formulation is withL-Glutamine and HEPES ans without Sodium Bicarbonate.

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium chloride (CaCl2) 154.52
Cupric sulfate (CuSO4-5H2O) 0.00125
Ferric nitrate (Fe(NO3)3-9H20) 0.05
Ferrous sulfate (FeSO4-7H2O) 0.417
Potassium chloride (KCl) 311.83
Magnesium chloride (MgCl2) 28.60
Magnesium sulfate (MgSO4) 48.83
Sodium chloride (NaCl) 6995.50
Sodium dihydrogen phosphate (NaH2PO4) 54.35
Sodium hydrogen phosphate (Na2HPO4) 71.06
Zinc sulfate (ZnSO4-7H2O) 0.432
OTHER COMPOUNDS
D-Glucose 3150.80
Hypoxanthine 2.715
Linoleic Acid 0.0421
Lipoic Acid 0.103
Phenol red 8.305
Putrescine-2HCl 0.0806
Sodium Pyruvate 55.00
HEPES 15mM
Thymidine 0.360
AMINO ACIDS
L-Alanine 4.45
L-Arginine hydrochloride 147.35
L-Asparagine monohydrate 7.51
L-Aspartic acid 6.66
L-Cysteine-HCl-H2O 17.56
L-Cystine dihydrochloride 31.29
L-Glutamic acid 7.36
L-Glitamine 365.10
Glycine 18.76
L-Histidine-HCl-H2O 31.48
L-Isoleucine 54.37
L-Leucine 58.96
L-Lysine hydrochloride 91.37
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.27
L-Serine 26.26
L-Threonine 53.56
L-Tryptophan 9.02
L-Tyrosine disodium 55.81
L-Valine 52.66
VITAMINS
Biotin 0.00367
D-Calcium pantothenate 2.12
Choline chloride 8.98
Folic acid 2.662
i-Inositol 12.51
Nicotinamide 2.018
Pyridoxal hydrochloride 2.00
Pyridoxine hydrochloride 0.031
Riboflavin 0.22
Thiamine hydrochloride 2.17
Vitamin B12 0.679