Sodium Dodecyl Sulfate (SDS) Solution (10%)

Sodium Dodecyl Sulfate Solution (10%) for SDS-Page
SKU: 01-890-1B

Availability: In stock

$35.00

Description

Details

Product Overview:

Sodium Dodecyl Sulfate (SDS) also known as Sodium Lauryl Sulfate is an anionic surfactant solution used as an adjuvant in lysing cells during DNA extraction (e.g. nucleic acid-protein complexes) and for unraveling proteins in SDS-Page.

SDS is probably not only the most researched anionic surfactant compound but also the most commonly used detergent in protein purification (e.g. DNA) and electrophoretic separation of proteins and lipids. It is used as a protein denaturing agent (PDA) during both extraction procedures and SDS-PAGE.

SDS-PAGE

Polyacrylamide Gel Electrophoresis (PAGE) is a technique in which the charge on the proteins results from their binding of SDS. Since the charge is proportional to the surface area of the protein, and the resistance to movement is proportional to diameter, small proteins migrate further. SDS is utilized in preparing proteins for electrophoresis in the SDS-PAGE technique. SDS is a compound that works by disrupting noncovalent bonds in the proteins, denaturing them, and causing the molecules to lose their conformation. The SDS anions bind to the main peptide chain at a ratio of one SDS anion for every two amino acid residues. This effectively imparts a negative charge on the protein that is proportional to the mass of that protein. The new negative charge is significantly greater than the original charge of that protein. The electrostatic repulsion that is created by the SDS binding causes the proteins to unfold into a different shape thereby eliminating the shape differences which is a factor in gel separation.

DSD Solution Features

    Anionic-detergent properties Liquid 1x concentrate Clear appearance solution Sterile

Specifications

Specifications

QTY 100 mL
Storage Conditions 2-8°C
Shipping Conditions Cold Pack

Documentation

Materials Safety Data Sheet

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