Osteogenic Differentiation

MSCgo™ Osteogenic XF Differentiation Media and the MSCgo™ Rapid Osteogenic XF Differentiation Media contain all the growth factors and supplements necessary for the directed differentiation of hMSCs. MSCgo™ Osteogenic XF was validated from various tissues including bone marrow (BM-hMSC), adipose tissue (AT-hMSC), and wharton jelly (WJ-hMSC).

Sample Data


Figure 1: Relative expression (RT-PCR) of osteocyte markers after 10 days of osteogenesis of hMSC using MSCgo™ Osteogenic XF. Osteogenic markers were upregulated whereas an undifferentiated hMSC marker (CD-105) was downregulated. BGLAP represents a maturation state of osteogenesis.


Figure 2: Positive Alizarin staining is observed, indicates of mature osteocytes after a 28 day differentiation assay of hMSC-AT using MSCgo™ Osteogenic XF medium


Figure 3: MSCgo™ Osteogenic XF led to highest expression of osteogenic markers and lowest expression of un-differentiated hMSC marker (CD-105) in comparison to commercial media. BGLAP represents a maturation state of osteogenesis.



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