MSC NutriStem® XF Medium

Defined, xeno-free, serum-free MSC culture medium

Eliminates the need to spend time and money prequalifying FBS lots
Name SKU Size Price Qty
MSC NutriStem® XF Medium (Basal Medium + Supplement) (US only) 05-200-1A-KT 500 mL
$310.00
MSC NutriStem® XF Basal Medium 05-200-1A 500 mL
$98.00
MSC NutriStem® XF Supplement Mix 05-201-1U 3 mL
$220.00

Description

Details

Product Overview:

Faster growth rates and stronger cell yields

MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium support long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.

hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media as well as other commercial serum-free media.  In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles and karyotypic stability over long-term culture. 

Key advantages

  • Serum-free, xeno-free, defined culture system
  • Superior cell growth compared to serum-containing media
  • Supports long-term growth and self-renewal of hMSCs from multiple sources
  • Consistent, reliable MSC cultures
  • Eliminates the need to spend time and money prequalifying FBS lots
  • GMP-defined, FDA Drug Master File (DMF) available

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

hMSC-BM were cultured in MSC NutriStem® XF


Figure: hMSC NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media.

Sample Data

Initial isolation

Figure: Human mesenchymal stem cells were initially isolated from Wharton's jelly (left image) and placenta (right image) using MSC NutriStem™ XF on pre-coated plates with MSC Attachment Solution (cat. no. 05-752-1). High number of hMSC was obtained.

Proliferation

Figure: MSC NutriStem® XF promotes excellent proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology.

 

Self-renewal potential

Figure: hMSC-BM and hMSC-AT were expanded in MSC NutriStem® XF for 3-5 passages prior to 14 days of CFU-F assay. Colonies were stained with 0.5% crystal violet (x100). The cells maintain their self-renewal potential.

Differentiation potential

Figure: hMSC-BM (top panel) and hMSC-AT (bottom panel) expanded in MSC NutriStem® XF for 3-5 passages prior to trilineage differentiation. Representative images of stained adipocytes (Oil Red O), osteocytes (Alizarin Red) and chondrocytes (Alcian Blue). The cells maintain their multilineage differentiation potential.

Specifications

Specifications

Form Liquid
Brand NutriStem®
Quality Control MSC NutriStem® XF Medium is application tested and validated for optimal maintenance and expansion of undifferentiated hMSCs, while maintaining their multi-lineage differentiation potential. Additional QC validation includes proper pH, osmolality, endotox
Specifications The complete mesenchymal stem cell culture media consist of two components: MSC NutriStem® XF Basal Medium (05-200-1) and MSC NutriStem® XF Supplement Mix (05-201-1). Individual components are not sold separately.
Instructions for Use MSC NutriStem® XF Medium is recommended for use in conjunction with MSC Attachment Solution (Cat. No. 05-752-1).

Storage and Stability

  • MSC NutriStem® XF Basal Medium (05-200-1) should be stored at 2 to 8°C. 
  • MSC NutriStem® XF Supplement Mix (05-201-1) should be stored at -20°C. Avoid freeze/thaw cycles.
  • The complete MSC NutriStem® XF Medium is stable at 2 to 8°C for up to 30 days.
  • Avoid exposure to light.  


Medium Preparation

  • Thaw frozen MSC NutriStem® XF Supplement Mix at at 2 to 8°C or room temperature
  • MSC NutriStem® XF Basal Medium contains L-glutmine
  • To prepare 100 mL of complete medium:  aseptically add 0.6 mL of MSC NutriStem® XF Supplement Mix to 100 mL of MSC NutriStem® XF Basal Medium
  • To prepare 500 mL of complete medium:  aseptically add 3 mL of MSC NutriStem® XF Supplement Mix to 500 mL of MSC NutriStem® XF Basal Medium
  • Store complete MSC NutriStem® XF Medium at 2 to 8°C protected from light for up to 30 days
Legal MSC NutriStem® XF Basal Medium is registered as an in-vitro diagnostic (IVD) medical device.

A Drug Master File (DMF) for MSC NutriStem® XF is available.

References

references

  1. C. Elseberg et al. The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech.
  2. C. Ceccaldi et al. Optimization of Injectable Thermosensitive Scaffolds with Enhanced Mechanical Properties for Cell Therapy. Macromolecular Bioscience, 2017
  3. D. Boruczkowski et al.Third-party Wharton’s jelly mesenchymal stem cells for treatment of steroid-resistant acute and chronic graft-versus-host disease: a report of 10 cases. Turkish Journal of Biology, 40: 493-500, 2016
  4. Jianxia H. et al. Long term effect and safety of Wharton's jelly-derived mesenchymal stem cells on type 2 diabetes. Experimental and Therapeutic Medicine, Volume 12 Issue 3, 2016
  5. S. Bobis-Wozowicz et al. Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles. Journal of Molecular Medicine, 2016
  6. L. Berger et al. Tumor Specific Recruitment and Reprogramming of Mesenchymal Stem Cells in TumorigenesisSTEM CELLS Volume 34, Issue 4, Version of Record online: 31 DEC 2015
  7. K.Y. Tan et al. Serum-free media formulations are cell line–specific and require optimization for microcarrier culture. Cytotherapy, 2015 
  8. Cai, Zhen, et al. Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia. Aesthetic plastic surgery 39.3 (2015): 431-439.
  9. A. Jarmalavičiūtė et al., Exosomes from dental pulp stem cells rescue human dopaminergic neurons from 6-hydroxy-dopamine–induced apoptosis. Cytotherapy, 2015
  10. M.Pokrywczynska et al., Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins. Archivum Immunologiae et Therapiae Experimentalis, May 2015
  11. U Pivoraitė et. al., Exosomes from Human Dental Pulp Stem Cells Suppress Carrageenan-Induced Acute Inflammation in Mice. Inflammation, April 2015
  1. S.H. Mei, et al. Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance. Cytotherapy, Volume 16, Issue 4, Supplement , Page S111, April 2014
  2. Li-Yi Sun, et al.Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants. Stem Cell Research & Therapy, 2014, 4:4
  3. C. Villeneuve, Optimisation de la production par génie tissulaire de tissus humains reconstruits in vitro pour une application clinique. Maîtrise en Biologie Cellulaire et Moléculaire Maître ès sciences (M. Sc.). Laval University, Québec, Canada
  4. Allen Kuan-Liang Chen, et al.Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction. Biotechnology Advances, Volume 31, Issue 7, 15 November 2013, Pages 1032–1046
  5. Mira Genser-Nir et al. Toward a serum-free, xeno-free culture system for optimal growth and expansion of hMSC suited to therapeutic applications. From 23rd European Society for Animal Cell Technology (ESACT), Meeting: Better Cells for Better Health.Lille, France. 23-26 June 2013
  6. McVey, Mark John, et al. Microparticles as biomarkers of lung disease-enumeration in biological fluids using lipid bilayer microspheres. American Journal of Physiology-Lung Cellular and Molecular Physiology (2016): ajplung-00369.
  7. Y. Lopez, et al. Identification of Optimal Conditions For Generating MSCs For Preclinical Testing: Comparison of Three Commercial Serum-Free Media And Low-Serum Growth Medium. From 18th ISCT Annual Meeting, Seattle, USA, 2012.
  8. R. D. Foster et al. GorodetskyFibrin microbeads loaded with mesenchymal cells support their long-term survival while sealed at room temperature.Tissue Eng Part C Methods2011TISSUE ENGINEERING: Part C, Volume 17, Number 7, 2011

Documentation

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Customer Reviews (1)

excellentReview by Jason
Quality
outstanding performance for my MSC cells, both morphology and proliferation (Posted on 8/4/2016)

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