DMEM - Dulbecco's Modified Eagle Medium

Many modifications of Eagle's Medium have been developed since the original formulation. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is considered one of the more common (e.g. MEM & RPMI) and less complex in comparison to enriched media which are utilized for more specialized cell types and are the basis for more unique serum-free media formulations. DMEM is a modification of Basal Medium Eagle (BME) which contains a higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for cultivation of certain cell types.

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