BME

Basal Medium Eagle (BME) originally developed by Harry Eagle, is a commonly used synthetic basal medium. BME according to the Tissue Culture Association refers only to several formulae developed by Eagle to support transformed HeLa cells in monolayer culture. HeLa denotes those epithelial tumor cells originally derived from the first continuously human cervical-cultured carcinoma strain propagated and later commercialized. Transformed cell lines are indicative of their almost infinite availability but with the caveat of having retained very few of its original in vivo characteristics. The morphology a cell line assumes is reflected upon the tissue from which it was derived. Cells derived from solid tissue (e.g., cervix, kidney, lungs) tend to grow as monolayers whereas cell lines derived from blood (e.g., leukemia’s, lymphomas) tend to grow in suspension. BME Earle's Salts supports the growth and viability of normal and transformed cell lines in monolayer culture.

Unlike the balanced salt solutions that form the basis of many complex media formulations and are utilized to maintain cells for the short term in a viable condition, BME is modified and enriched to promote the growth and viability of cells in culture. BME may be utilized for a broad variety of cell lines when properly supplemented.

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