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TAE Buffer 50X Conc.
TAE Buffer 50X Conc.
180.54 ש"ח

TAE Electrophoresis Buffer (50X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA) and as a running buffer for preparative work. Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation.

Also described in the literature is TAE's role in denaturing gradient gel electrophoresis methods for broad-range analysis and at various concentrations and to study the mobility of DNA in solution with and without NaCl1.

DNA mobility on AGE is known to depend on the composition and strength of electrode buffer as well as the agarose concentrations. The resolution of super coiled DNA is better in TAE than TBE but TAE's buffering capacity is rather low as it tends to become exhausted during successive electrophoresis. TAE buffer is more useful for larger DNA fragments (<2.0kb) but TBE is more effective to obtain higher resolution of smaller DNA fragments (i.e. 300bp). Either buffer is adequate is applicable for middle-sized DNA fragments.

  1. Take a bottle of TAE Electrophoresis Buffer (50X) from specified storage conditions at 15-30°C and read the label.
  2. Ensure that the cap of the bottle is tight.
  3. Gently swirl the solution in the bottle to ensure homogeneity.
  4. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
  5. Using aseptic/sterile technique under a laminar-flow culture hood and work according to established protocols.
  6. The TAE Electrophoresis Buffer Concentrate should be diluted to a working concentration of 1X before use.
  7. For each Electrophoresis, use fresh TAE Buffer at the working concentration of 1X.