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Description
Details
Product Overview
NutriStem® hPSC XF Medium is a widely published, defined, xeno-free, serum-free cell culture medium designed to support the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells. Protocols have been established around the world for applications ranging from derivation to differentiation.
NutriStem® hPSC XF Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, superior cell attachment and proliferation is observed with NutriStem® hPSC XF Medium aid high-throughput screening applications. NutriStem® hPSC XF Medium exhibits a consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation as well as increased reproducibility shown in long-term growth of over 50 passages.
Low-protein formulation that contains stable L-alanyl-L-glutamine and HSA.
NutriStem® hPSC XF Features
- Defined, serum-free, and xeno-free
- Flexible and compatible with multiple matrices
- Amenable to weekend-free culture
- FDA Drug Master File (DMF) available, produced under cGMP
- Enables efficient expansion and growth of hES and hiPS cells in feeder-free culture systems
- Extensively tested and widely used on multiple hES and iPS cell lines, including H1, H9.2, I6, I3.2, and CL1
Figure: 1 Human Embryonic Stem Cells (H1, passage 6) were seeded in 96-well plates (Matrigel coated) in BI's NutriStem® hPSC XF and competitor's media. Stem cell media were changed every 24 hours. Number of cells was determined using CyQuant™ cell proliferation assay kit.
Sample Data
Cell morphology
Figure: Normal Colony Morphology. H1 hES cells (top panel) and ACS-1014 hiPS cells (bottom panel) cultured in NutriStem® hPSC XF Medium on Matrigel-coated plates display colony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges.
Immunostaining
Figure: H1 cell morphology and immunofluorescence analysis of hESC markers red SSEA-4, green OCT4 and blue DAPI. H1 cells stained positive for the expression of pluripotency markers.
Embryoid body formation
Figure: Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem® hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm).
Taratoma formation
Figure: H9.2 hES cells were cultured for 11 passages in NutriStem® hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. The hES cells were subsequently injected into the hind leg muscle of SCID-beige mice for in vitro evaluation of pluripotency. The following tissues from all three germ layers were identified in H&E-stained histological sections of the teratoma 12 weeks post-injection: (A) cartilage (mesoderm), (B) epithelium (endoderm), and (C) neural rosette (ectoderm).
Specifications
Specifications
| Form | Liquid |
|---|---|
| Brand | NutriStem® |
| Storage Conditions | Store at -20ºC |
| Shipping Conditions | Dry Ice |
| Quality Control | NutriStem® hPSC is routinely tested for optimal maintenance and expansion of undifferentiated hESCs. Additional standard evaluations are pH, osmolality, endotoxins and sterility tests. |
| Instructions for Use |
Note: A common feeder-free basement membrane matrix is Matrigel, which is not xeno-free. Effective xeno-free alternatives to Matrigel is recombinant laminin, such as LaminStem(R) 521 (BI Cat. No. 05-753-1F) which has been validated to successfully culture human ES and iPS cells using NutriStem® hPSC XF medium. |
| Legal | NutriStem® hPSC XF is registered as an In-vitro diagnostic (IVD) medical device. NutriStem® is a registered trademark of Biological Industries. A Drug Master File (DMF) for NutriStem® hPSC XF is available. |
References
references
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- R. Ophir et al. Inflammation And Contractility Are Altered By Obstructive Sleep Apnea Children's Serum, In Human Embryonic Stem Cell Derived Cardiomyocytes. American Journal of Respiratory and Critical Care Medicine 2017
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- L. Jacquet et al. Three Huntington’s Disease Specific Mutation-Carrying Human Embryonic Stem Cell Lines Have Stable Number of CAG Repeats upon In Vitro Differentiation into Cardiomyocytes. PloS one 10.5, 2015
- V. Bellamy et al., Long-term functional benefits of human embryonic stem cell-derived cardiac progenitors embedded into a fibrin scaffold, The Journal of Heart and Lung Transplantation, 2014, in press
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Growing Methods of hESC and iPSC (Derivation, Expansion, Scaling up, and Suspensions)
Differentiation of Pluripotent Stem Cells
Cardiomyocyte differentiation
Gene Editing
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- StemAdhere™ Defined Matrix for hPSC. Primorigen Biosciences website.
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Different Basement Matrices
Induction of Pluripotency of hESC and iPSC
Proteins and Antibodies Expression and Isolation
Clinical Applications- Derivation and Expansion of hESC and IPSC
Drug Screening
Animal Models
Patents
Documentation
Material Safety Data Sheet
Manuals and Protocols
NutriStem® hPSC XF Medium Instructions for Use- NutriStem® hPSC XF Medium Technical Manual
- Weekend-free culture of human pluripotent stem cells on LN-521 (BioLamina)
Webinars & Videos
- On-Demand Webinar: Human Pluripotent Stem Cells (hPSC) – An Overview of Morphology and Culture Methods (BI-USA)
- On-Demand Webinar: Reprogramming and iPSC culture considerations for translational research (RegMedNet)
- Protocol video: Passaging Human Embryonic Stem Cells and Induced Pluripotent Stem Cells in NutriStem® XF/FF Culture Medium (Stemgent)
Product Literature
- NutriStem® hPSC XF Medium Product Information Sheet
- Case Study: Xeno-Free hiPS Cell Culture using NutriStem® hPSC XF Medium on a Human Vitronectin Substrate without the need for ROCK Inhibitors
- Application Note: Corning® PureCoat™ rLaminin-521 Cultureware: A Ready-to-use, Animal-free Vessel Platform for the Culture and Single Cell Passaging of Human Pluripotent Stem Cells (Corning)
- Application Note: NutriStem® hPSC XF Medium Supports Longterm Culture of Human Pluripotent Stem Cells (Corning)
- Application Note: Single Cell Passaging with NutriStem® hPSC XF on Laminin-521 without the Need for ROCK Inhibitor
- Technical Resources: Passaging Methods for hPSCs under Feeder-Free Conditions
- Technical Resources: Assuring Pluripotency of ESC and iPSC Lines
Frequently Asked Questions
Reviews
Customer Reviews (1)
- Five Stars, highly recommendedReview by John
-
Worked very well for us. High quality medium that gave us the most control over our cells. Ingredients you need. Highly recommended. (Posted on 10/24/2017)Quality
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