MSCgo™ Rapid Osteogenic Differentiation Medium

Complete, serum-free, xeno-free, ready-to-use medium

Optimized for faster directed differentiation of hMSCs to osteoblasts

SKU: 05-442-1B

Availability: In stock

$540.00

Description

Details

Product Overview

MSCgo™ Rapid Osteogenic Differentiation Medium is a serum-free (SF), xeno-free (XF), complete, and ready to use formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature osteocytes. The MSCgo™ Rapid Osteogenic Differentiation Medium is validated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC). The MSCgo™ Rapid Osteogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs.  The MSCgo™ Rapid Osteogenic Differentiation Medium enables fast osteogenesis if hMSCs.  Differentiation to mature osteocytes is seen in less than 10 days.

 

Features

  • Serum-free, xeno-free solution
  • Complete differentiation medium
  • Ready-to-use, simple protocol
  • Rapid osteogenic differentiation (less than 10 days)
  • Contains stable L-alanyl-L-glutamine
  • Does not contain antibiotics

 

Osteogenesis Results

Osteogenic differentiation of hMSC results in the formation of mineralized culture with calcified nodules and calcium secretion that can be detected with Alizarin Red S (ARS) staining. The ARS is used to stain calcium deposits formation which are an indication of mature osteocytes. The amount of calcified nodules formation and calcium secretion can be varying using different hMSC (e.g. source, age, and passage number).

Specifications

Specifications

QTY 100 mL
Form Liquid
Brand MSCgo™
Storage Conditions 2 to 8°C
Shipping Conditions Cold Pack
Quality Control The MSCgo Rapid Osteogenic Differentiation Medium is validated for optimal differentiation of hMSC into osteocytes. Additional tests are: pH, osmolality, endotoxins and sterility tests.
Specifications

Required Materials for Osteogenic Assay

  • MSCgo™ Osteogenic Differentiation Medium (BI Cat. No. 05-440-1) or MSCgo™ Rapid Osteogenic Differentiation Medium (BI Cat. No. 05-442-1)
  • MSC NutriStem® XF Medium (BI Cat. No. 05-200-1)
  • MSC Attachment Solution (BI Cat. No. 05-752-1)
  • Alizarin Red S (ARS) - OPTIONAL
Legal For human ex vivo tissue and cell culture processing applications. This reagent is not approved for human or animal use, or for application of in vitro diagnostic procedures.

Documentation

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