Vitronectin ACF (Human Recombinant)

Recombinant lyophilized protein for the attachment of human pluripotent stem cells.
SKU: 05-754-0002

Product Description

Details

Product Overview

Vitronectin is a secreted glycoprotein that can support cell adhesion through binding to various integrins and proteoglycans. Recombinant vitronectin ACF can function as a chemically-defined matrix component for the attachment of human embryonic and induced pluripotent stem cells in a feeder-free culture system. Recombinant Vitronectin ACF is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Vitronectin ACF is 52.2 kDa.

Additional Information

Additional Information

QTY 200 µg
Storage Conditions -20° to -80°C
Specifications Sterile filtered and lyophilized.
Authenticity: Verified by N-terminal and Mass Spectrometry analyses.
Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin: Endotoxin level is < 0.1 EU/μg of protein.
Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
Biological Activity: Promotes attachment of hPSC in serum-free conditions.
Instructions for Use

Preparation of Vitronectin ACF, 0.5mg/ml

Reconstitution procedure should be performed on ice.
  • Spin down the vial before reconstitution.
  • To the original 0.2mg vial add 0.4ml of sterile tissue culture water. DO NOT VORTEX.
  • Incubate on ice for a few minutes.
  • Gently mix by pipetting up and down.
  • Keep on ice for immediate use (up to 1 week at 2-8°C)
For long-term storage (3 months) aliquot and freeze at (-80)°C. Additional freeze-thaw cycles are not recommended.

Documentation

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