NutriStem® V9 XF Medium

Defined, xeno-free, serum-free culture medium for hPSC optimized for vitronectin
Name SKU Size
NutriStem® V9 XF Medium Basal Medium 05-105-1A 500 mL
NutriStem® V9 XF Supplement Mix 05-106-1F 1 mL

Product Description

Details

Product Overview

NutriStem® V9 XF medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA. It contains only the essential components required for long-term maintenance of hES and hiPS cells. NutriStem® V9 XF medium shows superior proliferation rates during long-term cultures, while maintaining the pluripotency of the cells.
NutriStem® V9 XF medium allows culture of hPSC in vitronectin pre-coated culture, as well as direct addition of vitronectin to the medium (no need for pre-coating).

Features

  • Maintains human ES and iPS cell pluripotency at high passage
  • Superior results using Vitronectin, may be used with Matrigel coated plates
  • Precoating-free option
  • Defined, serum-free, xeno-free
  • Produced under cGMP guidelines
  • Supports long-term expansion
  • Long weekend-free (up to 60 hours)
  • Cytokine- free basal medium, applicable for EBs formation, reprogramming, and differentiation
  • The proteins used: HSA (Human Serum Albumin), rh bFGF, rh TGFβ, human transferrin and recombinant human insulin.
  • Contains Alanyl glutamine. Does not contain antibiotics.
  • No need for ROCK Inhibitor


try it in your lab

Sample Data

Long-term Expansion
Long-term expansion of Hi hESC in NutriStem V9 Vs. E8

Nucleocounts performed on equal volume of cell suspension Chemometech, (count of aggregated cells) during long-term expansion of H1 cultured in NutriStem® V9 XF medium and E8 under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF. NutriStem® V9 XF medium shows superior proliferation rate in long-term culture (over 20 passages)


Cell morphology

Phase contrast images (x100) of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekendfree feeding regime, using 0.5μg/cm2 Vitronectin ACF. Representative images from culture at P6, P16 and P20. Undifferentiated colonies maintained during long-term culture of H1 hESC


Immunofluorescence staining
Tra 1-60
Immunofluorescence analysis of hESC cultured in NutriStem V9 - green stained
Nanog
Immunofluorescence analysis of hESC cultured in NutriStem V9 - red stained
Dapi
Immunofluorescence analysis of hESC cultured in NutriStem V9 - red stained
Merged
Immunofluorescence analysis of hESC cultured in NutriStem V9 - merged

Immunofluorescence analysis of human pluripotent markers of H1 hPSC expanded in NutriStem® V9 XF medium under a weekend-free feeding regime using Vitronectin ACF. Cells from P8 were fixated and stained for the classic pluripotent surface markers: TRA 1-60 (Alexa Fluor) (green) and nuclear conjugated markers: Nanog-RRX, counterstained with DAPI (blue). Scale bar 200μm. Cells cultured in NutriStem® V9 XF Medium on vitronectin express high levels of pluripotent markers


Flow cytometric quantification

Immunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium

Immunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF at P8 and P17. Data presented as % expression from gated viable cells. High expression of pluripotent stem cell markers in cells cultured in NutriStem® V9 XF medium on vitronectin

Additional Information

Additional Information

QTY 500 mL
Form Liquid
Brand NutriStem®
Storage Conditions Store at -10° to -20°C
quality control Each lot is tested for performance using H1 hESC by expansion of the cells for 3 passages, IF staining for pluripotency marker, and morphology. Other tests include sterility, pH, osmolality and endotoxins levels.
legal NutriStem® is a registered trademark of Biological Industries​.

Documentation

Reviews

Write Your Own Review

You're reviewing: NutriStem® V9 XF Medium

How do you rate this product? *

  1 star 2 stars 3 stars 4 stars 5 stars
Quality

Product Tags

Product Tags

Use spaces to separate tags. Use single quotes (') for phrases.

You may also be interested in the following product(s)