Description
Details
Product Overview
MSCgo™ Adipogenic Differentiation Medium is a serum-free and xeno-free formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature adipocytes. The MSCgo™ Adipogenic Differentiation Medium is validated to efficiently differentiate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC), and Wharton’s jelly (WJ-MSC). The MSCgo™ Adipogenic Differentiation Medium kit includes a basal medium and two supplement mixes, containing all growth factors and supplements necessary for a complete adipoogenic differentiation medium. No additional supplements are required.
The MSCgo™ Adipogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs. This kit enables reliable differentiation of hMSCs to mature adipocytes without background differentiation or interruption in cellular metabolism.
Features
- Serum-free, xeno-free medium
- All required growth factors and supplements included in kit
- Reliable differentiation to mature adipocytes
- Each lot is application tested
- Contains stable L-alanyl-L-glutamine
- Does not contain antibiotics
Components
- MSCgo Adipogenic Differentiation Basal Medium: 100 mL
- MSCgo Adipogenic Differentiation Supplement Mix I: 100 μL
- MSCgo Adipogenic Differentiation Supplement Mix II: 1.5 mL
Adipogenesis Results
Adipogenic differentiation of hMSC results in the formation of spherical cells accumulated with lipid droplets that can be detected using an inverted microscope. The amount of differentiated cells can be vary using different hMSC (e.g. source, age, and passage number).
After expansion in culture using MSC NitriStem XF Medium, hMSCs from adipose tissue (hMSC-AT), bone marrow (hMSC-BM), and cord tissue (hMSC-CT) were transfered to a differentiation assay in MSCgo™ Adipogenic Differentiation Medium. Images were taken after 16 days of adipogenesis and Oil Red-O staining (20x).
Specifications
Specifications
QTY | 100 mL |
---|---|
Form | Liquid |
Brand | MSCgo™ |
Storage Conditions | Adipogenic Differentiation Basal Medium: 2 to 8°C Adipogenic Differentiation Supplement Mix I: -20°C Adipogenic Differentiation Supplement Mix II: -20°C |
Shipping Conditions | Cold Pack |
Quality Control | The MSCgo Adipogenic Differentiation Medium is validated for optimal differentiation of hMSC into chondrocytes. Additional tests are: pH, osmolality, endotoxins and sterility tests. |
Specifications | Required Materials for Adipogenic Assay:
|
Instructions for Use | Complete Medium Preparation
Note: The combination of both MSCgo Adipogenic Supplement Mix I and II into the Basal Medium generates a complete medium that is ready for use. No additional supplements are required. |
Legal | For human ex vivo tissue and cell culture processing applications. This reagent is not approved for human or animal use, or for application of in vitro diagnostic procedures. |
References
references
- J. Leber et al., Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media. Process Biochemistry, volume 59, Part B, August 2017, Pages 255-265
- L. Pu et al., Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration. Stem Cell Research & Therapy 2017 8:72
- M. Meng et al., Umbilical cord mesenchymal stem cell transplantation in the treatment of multiple sclerosis. American Journal of Translational Research, 2018;10(1):212-223
Documentation
Materials Safety Data Sheet
- MSCgo™ Adipogenic Differentiation Medium (MSDS)
- MSCgo™ Adipogenic Differentiation Supplement Mix I (MSDS)
- MSCgo™ Adipogenic Differentiation Supplement Mix II (MSDS)
Manuals and Protocols
Product Literature
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.