Mouse Embryonic Stem Cell Basal Medium

Basal medium designed for the growth of mouse embryonic stem cells (ES)
SKU: 01-171-1A

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Product Overview

Mouse embryonic stem cells are pluripotent cells derived from the inner cell mass of the blastocyst. Undifferentiated embryonic stem cells can be maintained in-vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst. Mouse Embryonic syem cells may differentiate in-vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors. General culture conditions are well established and usually require embryonic stem cells to be grown on an inactive feeder cell layer or on gelatin-coated plates with Leukemia Inhibitory Factor (LIF) in the stem cell culture medium.
Mouse Embryonic Stem Cell Basal Medium has been optimized to grow and maintain undifferentiated mouse embryonic stem cells. The medium may be used with the addition of Fetal Bovine Serum (FBS) or with any serum replacement designed for mouse embryonic stem cells.
The medium contains L-glutamine in a stable form.

Additional Information

Additional Information

QTY 500 mL
Form Liquid
Storage Conditions Store at 2-8°C
Instructions for Use

Before use, the medium should be supplemented with the following:

  1. Fetal Bovine Serum (FBS), 10% or serum replacement
  2. Mercaptoethanol, 0.1mM
  3. LIF, 1000units/ml: only if gelatin-coated plates are used
  4. Antibiotics (if desired). If serum replacement is used, it is recommended to use a lower concentration of antibiotics.

The medium can be used with feeder layer dependent ES cell lines or with feeder independent ES cell lines using gelatin-coated plates, See: 0.1% gelatin, Cat. No. 01-944-1

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