CryoStem™ Freezing Medium

Chemically defined, animal component-free, protein-free cryopreservation solution for human ES cells and iPS cells.
Name SKU Size
CryoStem™ Freezing Medium 05-710-1D 10 mL
CryoStem™ Freezing Medium 05-710-1E 50 mL

Product Description

Details

Product Overview

CryoStem Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.

CryoStem contains methylcellulose and DMSO in place of serum.  The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.  

CryoStem Features

  • Chemically defined, Animal component-free (ACF), Protein-free
  • Works with various media
  • Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
  • High recovery efficiency: maintains excellent attachment ability as well as growth performance
  • Maintains hESC and hiPSC pluripotency
  • Complete formulation; Ready-to-use at 2-8°C

Alkaline Phosphatase (AP) staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem freezing medium. Cells were maintained on feeder layer. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

AP staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem freezing medium. Cells were maintained in feeder-free conditions. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

H1 cell morphology and immunofluorescence analysis of hESC marker SSEA-4 at Passage 2, recovered from freezing condition in CryoStem freezing medium.

H1 hESC BGO1V/hOG cels (Variant hESC hOct4-GFP reporter cells)

1 hour post-thawing

Day 1 Post-thawing

Day 4 Post-thawing

hESC were frozen in CryoStem and were thawed into NutriStem® hESC XF on Matrigel.

Additional Information

Additional Information

Form Liquid
Brand CryoStem™
Storage Conditions Store at 2 - 8ºC​
Instructions for Use Do not use if a visible precipitate is observed in the medium. Do not use beyond the expiration date indicated on the product label.
legal CryoStem Freezing Medium is intended for in vitro diagnostic use

References

references

Documentation

Reviews

Customer Reviews (1)

Great product for stem cell long term freezing!Review by Bar Philosof
Quality
I use it to freeze cancer stem-like cell, and it is a great product. (Posted on 20/11/2017)

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