IMDM, HEPES

with L-Glutamine, 500 mL
SKU: 01-058-1A

Availability: In stock

$37.00

Description

Details

Product Overview:

IMDM (Iscove's Modified Dulbecco's Medium) is utilized not only for more specialized cell types but also as the basis for some of the more unique serum-free media formulations. The original formulation designed by Iscove and associates, demonstrated that erythrocytes and macrophage precursor cells (e.g. primary Haemopoietic Cells) could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. IMDM is a modification of DMEM (Dulbecco's Modified Eagle's Medium) containing selenium, additional AA's, vitamins and other components. IMDM is a highly enriched synthetic media well-suited for rapidly proliferating and high-density cell cultures in a 5% CO2 atmosphere.

IMDM medium reportedly supports Murine B Lymphocytes, Haemopoietic tissues from Bone Marrow, B-cells stimulated with Lipopolysaccharide, and also T-Lymphocytes and a wide variety of Hybrid cells. When utilized in conjunction with serum, it supports a broad spectrum of Mammalian cell types including but not exclusive to bone marrow, hybridomas and lymphocytes.



This IMDM is modified as follows:

With

  • L-Glutamine
  • Phenol Red
  • Sodium Bicarbonate
  • HEPES

Without:

    • Beta Mercaptoethanol
    • Alpha-Thioglycerol

The complete formulation is available.

Specifications

Specifications

Glutamine L-Glutamine
QTY 500 mL
Storage Conditions 2-8°C
Form Liquid
HEPES Buffer HEPES
Sodium Bicarbonate Buffer Sodium Bicarbonate
Sodium Pyruvate Additive with Sodium Pyruvate

Documentation

Materials Safety Data Sheet

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