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EZ-RNA Total RNA Isolation Kit

Solvent-based, scalable, ready-to-use
SKU: 20-400-100

Description

Details

Product Overview

EZ-RNA Total RNA Isolation Kit is a complete kit with ready-to-use reagents for the extraction of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. It is based on disruption of cells in guanidine thiocyanate/detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and allows simultaneous processing of a large number of samples. The resulting RNA is suitable for the isolation of Poly A+ RNA or for Northern Blotting, Dot Blotting, in vitro Translation, Molecular Cloning, RT-PCR and RNase Protection Assays, or other analytical procedures.

DNA and proteins can also be recovered from the interphase and the organic phase of the same sample.

RNA Isolation Kit Advantages

  • Ready-to-use reagents
  • Solvent-based
  • Scalable
  • Working with variant samples: human, animal, yeast and viral origin
  • Extraction of RNA, DNA and proteins from the same sample
  • Results in less than 1 hour

Note: for RNA Isolation Kit without chlorophorm please refer to EZ-RNA II Total

Specifications

Specifications

QTY 100 mL
Storage Conditions 2-8°C
Shipping Conditions Cold Pack

References

references

  1. N. Wallis, et al. Small molecule inhibitor of Igf2bp1 represses Kras and a pro-oncogenic phenotype in cancer cells. RNA Biology, (2021) DOI: 10.1080/15476286.2021.2010983
  2. S. M. Najimudeen, et al. Pathogenesis and host responses in lungs and kidneys following Canadian 4/91 infectious bronchitis virus (IBV) infection in chickens. Virology, 2021, https://doi.org/10.1016/j.virol.2021.11.013.
  3. A. Moshe, et al. Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation. Cells, Volume 9 Issue 7 10.3390/cells9071683  
  4. L. Cohen, et al. Cardiac remodeling secondary to chronic volume overload is attenuated by a novel MMP9/2 blocking antibody. PLoS ONE 15(4): e0231202. https://doi.org/10.1371/journal.pone.0231202
  5. Y. Lampert, et al. Indigenous versus Lessepsian Hosts: Nervous Necrosis Virus (NNV) in Eastern Mediterranean Sea Fish. Viruses 2020, 12, 430; doi:10.3390/v12040430
  6. L. Zach, et al. The effects of Rapamycin on Telomerase Activity and Regulation in Cancer Cells Br J Cancer Res 2019,2:4, 334 - 340. doi: 10.31488/bjcr.140
  7. T. Shimatani, et al. Neurally adjusted ventilatory assist mitigates ventilator-induced diaphragm injury in rabbits Respir Res 20, 293 (2019) doi:10.1186/s12931-019-1265-x
  8. S. Cohen Site-selective modification of polysaccharides and applications thereof
  9. E. Bigelman et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLOS One, https://doi.org/10.1371/journal.pone.0198196, 2018
  10. O. Stein et al. The tomato cytosolic fructokinase FRK1 is important for phloem fiber development. Biologia Plantarum, June 2018, Volume 62, Issue 2, pp 353–361
  11. R. Kedmi et al. A modular platform for targeted RNAi therapeutics. Nature Nanotechnology, volume 13, pages 214–219 (2018)
  12. E. Kaplan et al. Enhanced Bacterial Fitness Under Residual Fluoroquinolone Concentrations Is Associated With Increased Gene Expression in Wastewater-Derived qnr Plasmid-Harboring Strains. Front Microbiol. 2018; 9: 1176.
  13. S.B Harosh-Davidovich and I. Khalaila O-GlcNAcylation affects β-catenin and E-cadherin expression, cell motility and tumorigenicity of colorectal cancer. Experimental Cell Research, Volume 364, Issue 1, 1 March 2018, Pages 42-49
  14. A. Gelgor et al. Nucleolar stress enhances lytic reactivation of the Kaposi’s sarcoma-associated herpesvirus. Oncotarget. 2018 Mar 2; 9(17): 13822–13833.
  15. R. Bar et al. The effects of apolipoprotein E genotype, α-synuclein deficiency, and sex on brain synaptic and Alzheimer's disease–related pathology. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring, Volume 10, 2018, Pages 1-11

Documentation

 

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.

COA's can be downloaded from Sartorius's Certificates Portal.

For certificates issued before November 15, 2021, please enter below the product lot number and click search.
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Reviews

Customer Reviews (1)

Good productReview by Yamit Bar Lev
Quality
Similar to other phenol based isolation methods, good yield and cleaning (Posted on 7/4/2018)

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