Crystalline Trypsin Solution (0.02%)

Non-cytotoxic, Enhanced cell viability
Name SKU Size Price Qty
Crystalline Trypsin Solution (0.02%) 03-047-1A 500 mL
$74.00

Description

Details

Product Overview

Crude trypsin is often the subculturing agent of choice for cell dissociation/ disaggregation of adherent cells, although the treatment may be cytotoxic if prolonged. Over-trypsinization is a common cause of subculture problems. When using crystalline trypsin versus crude trypsin forms, crystalline trypsin does not damage cells after prolonged exposure.

Advantages

  • Does not require multiple-change procedures
  • Maintains better cell viability and enhances the process of cell passaging
  • Not as cytotoxic to cells with all the negative ramifications of crude trypsin
  • Contains additives that protect the cell wall, enhancing cell viability, and it is a ready-to-use solution

The complete formulation is available.

Specifications

Specifications

Storage Conditions -20°C
Specifications
  • Cells must NEVER remain in the crude trypsin for longer than 3-5 minutes as they may be seriously damaged in the process (i.e. damage to the intracellular proteins).
  • Cells should NEVER be left without a fluid layer.
  • The use of crystalline trypsin, rather than crude trypsin, most often performs better long-term cell growth in serum-free medium formulations. It is specifically formulated to have a gentle nature with much better cell viability, in which the cells are not subject to the vagaries of time and circumstance as when the cruder forms of trypsin are utilized.

Documentation

Materials Safety Data Sheet

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