CryoStem Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.
CryoStem contains methylcellulose and DMSO in place of serum. The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.
- Chemically defined, Animal component-free (ACF), Protein-free
- Works with various media
- Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
- High recovery efficiency: maintains excellent attachment ability as well as growth performance
- Maintains hESC and hiPSC pluripotency
- Complete formulation; Ready-to-use at 2-8°C
Alkaline Phosphatase (AP) staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem freezing medium. Cells were maintained on feeder layer. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.
AP staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem freezing medium. Cells were maintained in feeder-free conditions. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.
|Storage Conditions||Store at 2 - 8ºC|
|Instructions for Use||Do not use if a visible precipitate is observed in the medium. Do not use beyond the expiration date indicated on the product label.|
|Legal||CryoStem Freezing Medium is intended for in vitro diagnostic use|
- N. Nishishita et al. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. American journal of stem cells 4.1 (2015): 38.
- C. Martin et al. Highly Efficient microRNA-Enhanced mRNA Reprogramming of Diseased Human Fibroblasts in a Feeder-Free Culture System. STEMGENT Application Note, 2014
- C. Buensuceso et al. Induced pluripotent stem cells prepared from human kidney-derived cells . US Patent 20140073049 A1, 2014
- R. Sivapatham, X.Zeng. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods in Molecular Biology, 2014
- L. Tian, N. Prasad, Y. Jang. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2014
- C. Buensuceso et al. Induced pluripotent stem cells from human umbilical cord tissue-derived cells. US Patent 20130157365, 2013
- S. Hikita et al. Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation. US Patent 20130196369 A1, 2013
- N. Nishishita et al. Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State. PLoS ONE 7(6): e38389. doi:10.1371/journal.pone.0038389 , 2012
- F. Pistollato et al. Standardization of pluripotent stem cell cultures for toxicity testing. Vol. 8, No. 2 , Pages 239-257 (doi:10.1517/17425255.2012.639763), 2012