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BIO-MPM-1 SFM

Serum-free medium for a wide variety of anchorage-dependent cells
4 x 500 mL
SKU: 05-060-1A

Description

Details

Product Overview:

BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. After the addition of 2mM glutamine, BIO-MPM-1 Medium is ready to use for adherent cells. The formulation contains no albumin, which has been found to be non-essential for cell growth, and even prevents efficient adhesion in some cases. The protein content of BIO-MPM-1 is therefore less than 30 mg/L, and the medium contains no growth factors or hormones other than insulin. The formulation does not contain an attachment factor, which in many (but not all) cases must be added for successful use.

BIO-MPM-1 Medium is suitable for a wide variety of cells. The following are some of the cells which have been cultured with BIO-MPM-1:

  • HUVECs
  • MDCK cells
  • HEK293
  • Murine aortic cells (Aortic ring assay)
  • Human chondrocytes
  • Human placental trophoblast cells
  • Rat heart cells and cardiac fibroblasts
  • Chicken retinal cells
  • Avian epiphyseal chondrocytes
  • Human NK cells
  • Murine neonatal neurons
  • Starfish epithelial cells
  • HK2 cells

Specifications

Specifications

QTY 4 x 500 mL
Storage Conditions 2-8°C
Shipping Conditions Cold Pack

References

references

  1. M. Esteve, et al. Spectrum of gluten-sensitive enteropathy in first-degree relatives of patients with coeliac disease: clinical relevance of lymphocytic enteritis. Gut 2006;55:1739–1745. doi: 10.1136/gut.2006.095299
  2. S. Ben-Sasson, H. Reuveni, Pro-angiogenic peptides and peptide conjugates. US Patent, US9873724B2, 2018
  3. J. Elayyan et-al. LEF1-mediated MMP13 gene expression is repressed by SIRT1 in human chondrocytes. The FASEB Journal, Vol. 31, No. 7, July 2017
  4. L. Ben-Aderet et-al. Detecting cathepsin activity in human osteoarthritis via activity-based probes. Arthritis Research & Therapy 2015
  5. Z. Aberman, Adherent cells from placenta and use of same in disease treatment. US Patent, US9175262B2, 2015
  6. J.P. Li et al. Heparanase deficient non-human mammals. US Patent, US8809620B2, 2014
  7. E. Anuka et-al. Infarct-Induced Steroidogenic Acute Regulatory Protein: A Survival Role in Cardiac Fibroblasts. Molecular Endocrinology, Volume 27, Issue 9, 1 September 2013, Pages 1502–1517
  8. R. Seger, G. Maik-Rachline, Variants of pigment epithelium derived factor and uses thereof. US Patent US8530416B2, 2013
  9. Avraham-Davidi I, et al,. On-site education of VEGF-recruited monocytes improves their performance as angiogenic and arteriogenic accessory cells. The Journal of Experimental Medicine, Vol. 210 No. 12 2611-2625 (2013)
  10. Cerdeira A S, et al., Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined FactorsThe Journal of Immunology, 190:3939-3948 (2013).
  11. Gorovits R, Avidan N, Avisar N, et al., Glutamine synthetase protects against neuronal degeneration in injured retinal tissue. Proc. Natl. Acad. Sci. USA, 94: 7024-7029 (1997).
  12. Monsonego E, Baumbach RW, Lavelin I, et al., Generation of growth hormone binding protein by avian growth plate chondrocytes is dependent on cell differentiation.  Molecular and Cellular Endocrinology 135: 1-10 (1997)
  13. Jacob Hanna, et al., Decidual NK cells regulate key developmental processes at the human fetal-maternal interface. Nature (Medicine) On line (2006).
  14. Grunwald Myriam, et al., VEGF-produced adult neovascularization. Recruitment, retention and role of accessory cells. Cell 124 (1) : 175-189 (2006).
  15. Ben-Yosef, et al., Hypoxia of endothelial cells leads to MMP-2-dependent survival and death. Am. J. Physiol. Cell Physiol. 289 (1) : 1321-1331 (2005).
  1. T Licht, L Tsirulnikov, H Reuveni, T Yarnitzky et al. Induction of proangiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3). Blood 102 (6): 2099-2107 (2003).
  2. Niv Masha, et al., Sequence based design of kinase inhibitors applicable for therapeutics and target identification. J. Biol. Chem. 279 (2): 1242-1255 (2004).
  3. V Shneyvays, D Leshem, T Zinman et al., Role of adenosine A1 and A3 receptors in regulation of cardiomyocyte homeostasis after mitochondrial respiratory chain injury. Am. J. Physiology 288: H2792-H2801 (2005).
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  10. O Turowska, A Nauman, M Pietrzak, P Poplawski, et al., Overexpression of E2F1 in Clear Cell Renal Cell Carcinoma: A Potential Impact of Erroneous Regulation by Thyroid Hormone Nuclear Receptors. Thyroid vol. 17(11): 1039-1048 (2007).
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Documentation

Materials Safety Data Sheet

Manuals and Protocols

Certificate of Analysis

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