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BIO-AMF™-2 Medium

Optimized for the primary culture of human amniotic fluid cells and chorionic villi (CV) samples used in prenatal diagnostic testing

Will be discontinued from 1/10/2022
Name SKU Size
BIO-AMF™-2 Medium 01-194-1A 500 mL
BIO-AMF™-2 Medium 01-194-1B 100 mL

Description

Details

Product Overview:

BIO-AMF™-2 Complete Medium is a fully-supplemented medium optimized for the primary culture of human amniotic fluid and chorionic villi cells for cytogenetic studies.

Features

  • Ready-to-use We offer a single bottle formulation, complete with L-Glutamine and Gentamycin, which is shipped frozen. Upon receipt, simply thaw and use. We recommend storing thawed medium at 2-8°C for up to seven days.
  • Quality and performance testing BIO-AMF-2 Complete Medium is evaluated for cell growth using primary human amniotic fluid cells from a leading clinical cytogenetics laboratory. Additionally we test each batch for sterility, pH, osmolality and endotoxin concentrations.
  • Optimized and complete solution BIO-AMF™-2 Complete Medium is prepared with Fetal Bovine Serum (FBS), L-Glutamine and Gentamycin optimized for both open (CO2) and closed systems.

    cGMP Manufacturing and Quality System BIO-AMF™-2 Complete Medium is manufactured at our cGMP compliant facility, located in Beit Haemek, Israel. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

Brand BIO-AMF™
Form Liquid
Cell Type Amniocytes
Glutamine L-Glutamine
Antibiotics Gentamycin
Shipping Conditions Dry Ice
Storage Conditions -20ºC
Shelf Life 24 months from date of production and stored frozen
After thawing, 7 days at 2°C to 8°C

References

references

  1. T. Eigler, et al. ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion. Developmental Cell, 2021, https://doi.org/10.1016/j.devcel.2021.11.022.
  2. F. Martello, et. al. Generation of an induced pluripotent stem cell line (UCSCi001-A) from a patient with early-onset amyotrophic lateral sclerosis carrying a FUS variant. Stem Cell Research, 2021, https://doi.org/10.1016/j.scr.2021.102461.
  3. S. Neeman-Egozi , et al. Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs. In: . Methods in Molecular Biology. Springer, New York, (2021) https://doi.org/10.1007/7651_2021_387
  4. S. Lattante, et al. Novel variants and cellular studies on patients’ primary fibroblasts support a role for NEK1 missense variants in ALS pathogenesis. Human Molecular Genetics, 2021,  https://doi.org/10.1093/hmg/ddab015
  5. R. Gomez,  et al. Genetic findings in miscarriages and their relation to the number of previous miscarriages. Arch Gynecol Obstet (2020). https://doi.org/10.1007/s00404-020-05859-x
  6. Li S.,et al. Prenatal diagnosis of rearrangements in the fetal 22q11.2 region. Mol Cytogenet 13, 28 (2020). https://doi.org/10.1186/s13039-020-00498-y
  7. N. Romano, et al. ALS skin fibroblasts reveal oxidative stress and ERK1/2-mediated cytoplasmic localization of TDP-43. Cellular Signalling Volume 70, June 2020, https://doi.org/10.1016/j.cellsig.2020.109591
  8. N.Y.Choi, et al. Generation of human androgenetic induced pluripotent stem cells. Sci Rep 10, 3614 (2020). https://doi.org/10.1038/s41598-020-60363-1
  9. Z. Miao et al. Combined use of bacterial artificial chromosomes-on-beads with karyotype detection improves prenatal diagnosis. Molecular Cytogenetics 2019 12:9
  10. Constantinou M, Kałuzewski B, Helszer Z, et al., Prenatal detection of maternal UPD15 in a new case with i(15p) by Timing Replication Test (TRT) and methylation analysis. J. Appl. Genet., 44(2): 209-218 (2003).
  11. Sarig R, Fuchs O, Tencer L, Panski A, Nudel U, et al. Cloned Myogenic Cells Can Transdifferentiate In Vivo into Neuron-Like Cells. PLoS ONE 5(1) (2010).
  12. M. Krkavcová, N. Jančárková, M. Janashia, et al., Genetic alterations in gynecological malignancies. NEOPLASMA 55 (3) (2008).
  13. E. Guetta, G. Barkai, L. Gutstein-Abo.  Trophoblasts Isolated from the Maternal Circulation : In Vitro Expansion and Potential Application in Non-invasive Prenatal Diagnosis. Journal of Histochemistry and Cytochemistry 53 (3): 337-339 (2005).
  14. J.M. Bellón, A. Bajo, N. Ga-Honduvilla, et al., . Fibroblasts From the Transversalis Fascia of Young Patients With Direct Inguinal Hernias Show Constitutive MMP-2 Overexpression. Annals of Surgery. 233(2): 287–291 (2001).
  15. U. Ozgen , M. Ikbal, A. Hacimuftuoglu, et al., Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots. Journal of Ethnopharmacology 104 (1-2, 8): 100-103 (2006).
  1. R. Sarig, Z. Baruchi, O. Fuchs, et al., Regeneration and Transdifferentiation Potential of Muscle-Derived Stem Cells Propagated as Myospheres. Stem Cells 24 (7): 1769-1778 (2006).
  2. Ji Hyeon Park, Jung Hoon Woo, Sung Han Shim, et al., Application of a target array Comparative Genomic Hybridization to prenatal diagnosis. BMC Medical Genetics 11:102 (2010).
  3. S. Han Shim, T. Ki Yoon, D. Hyun Cha, et al., Endothelial Nitric Oxide Synthase (eNOS) gene polymorphisms in spontaneously aborted embryos. Genes & Genomics 32 (3) (2010).
  4. S. Ayesh, I. Farrah, T. Schneider, et al., The involvement of H19 non-coding RNA in stress: Implications in cancer development and prognosis. Gene Therapy and Molecular Biology 8: 403-412 (2004)
  5. Y. Gruenbaum-Cohena, I. Harelb, K.B. Umanskya, et al., The actin regulator N-WASp is required for muscle-cell fusion in mice. PNAS July 10 2012 Vol 109 n 28 11211-11216.
  6. C. Mozzetta, S. Consalvi, V. Saccone, et al., Fibroadipogenic progenitors mediate the ability of HDAC inhibitors to promote regeneration in dystrophic muscles of young, but not old Mdx mice. EMBO Molecular Medicine Apr 2013; 5(4): 626–639

Documentation

Materials Safety Data Sheet

Manuals and Protocols

Product Information

Certificate of Analysis

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