Accutase Solution

Gentle alternative solution to trypsin

Used for cell detachment and tissue dissociation

SKU: 03-073-1B

Availability: In stock

$194.00

Description

Details

Product Overview

Accutase Solution is an alternative solution to trypsin developed for gentle and effective detachment of adherent cells.  

Accutase is mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated culture dishes as well as for gentle tissue dissociation.  The well-balanced combination of proteolytic and collagenolytic activities ensures that surface proteins and epitopes stay entirely intact. Accutase is therefore perfectly suited for applications that require an unchanged cell surface, such as detaching cells for analysis of cell surface markers, cell proliferation, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Accutase has been shown to be effective on a number of cell types, including human embryonic stem (hES) cells, neuronal stem cells, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, and macrophages.

 

Features

  • Ready-to-use solution
  • Gentle formula for cell detachment and tissue dissociation
  • No mammalian or bacterial-derived components
  • Effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells
  • Ideal for detaching cells for cell surface marker analysis, virus growth assay, flow cytometry, and bioreactor scale-up

Specifications

Specifications

QTY 100 mL
Storage Conditions -20ºC
Shipping Conditions Dry Ice
Form Liquid

References

references

1.  Bajpai, R., et al. (2008). Efficient propagation of single cells accutase-dissociated human embryonic stem cells. Mol Reprod Dev 75: 818-827.

2.  Zheng, K., et al. (2006). Comparison of different culture modes for long-term expansion of neural stem cells. Cytotechnology 52: 209-218.

3.  Wachs, F.P., et al. (2003). High efficacy of clonal growth and expansion of adult neural stem cells. Lab Invest. 83: 949-962.

Documentation

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